NEERI finds unique method of RT-PCR test for Covid-19 test, no swab, cheap, simple and quick result

New Delhi: Since the outbreak of the global pandemic Covid-19, India has been taking several steps to enhance the infrastructure and capacity to test it. Scientists at the National Environmental Engineering Research Institute (NEERI), Nagpur, under the Council of Scientific and Industrial Research (CSIR), have created another record in this link, under which ‘salt’ for testing samples of Covid-19 (Saline Gargle) RT-PCR method has been found with the water of water.

Many benefits from one method: simple, fast, comfortable and economical
This method of salt water gagare (Céline Gargle) brings many benefits together. This method is simple, fast, cost-effective, patient-friendly and comfortable and also results quickly. Given the minimum infrastructure requirements.

This method is suitable for rural and tribal areas. Speaking to the Press Information Office, Dr. Krishna Khairnar, Senior Scientist, Environmental Virology Cell at the National Environmental Engineering Research Institute (NEERI), said: “The swab collection method requires time.

In addition, since the infected infected with this technique can also face some discomfort during the investigation, they can sometimes be uncomfortable. Also, the samples collected in this way take some time to be taken to the collection center and testing center. On the other hand, RT-PCR method of gargle (saline gargle) with salt water is immediate, comfortable and patient friendly. The sample is taken immediately and the results will be available within three hours. ”

The patient can collect his own sample
According to Dr. Khairnar, this method is non-invasive and so simple that the patient can collect the sample himself. He stated that, “Collection methods such as collecting nasopharyngeal and oropharyngeal swabs by the nose and by mouth require technical expertise and also take time. In contrast, RT-PCR of salt water gargle (Céline Gargle) The method uses a simple collection tube filled with salt water (saline water).

The patient gargles with this solution and pours it inside the tube. This sample in the collection tube is transported to the laboratory where it A special buffer solution (solution) is prepared by NEERI at room temperature. On heating this solution, an RNA template is prepared, which is further processed for reverse transcription polymerase chain reaction (RT-PCR).

This particular method of collecting and processing a sample enables us to use it in place of other expensive structural requirements of RNA extraction. People can also test it themselves, because this method can use its own sample ( Sample) can be taken by itself. ” This method is also environmentally friendly, as it has minimal waste production.

Boon for testing in rural and tribal areas
Scientists hope that this unique testing technique will prove particularly beneficial for rural and tribal areas where infrastructure requirements may yet pose an obstacle. This non-technology has received the approval of the Indian Council of Medical Research (ICMR). Also NEERI has been asked to arrange the necessary training trained to other testing laboratories to help in its use across the country.

The Nagpur Municipal Corporation has given permission to proceed this process, after which the testing has started as per the accepted testing protocol in NEERI.

“Need to implement across India”
Scientists, researchers and lab-technicians of the Environmental Virology Cell (Environmental Virology Cell) at NEERI have made untiring efforts to develop this patient-friendly technology amid growing Covid-19 infections in the Vidarbha region. Dr. Khairnar and his team hope that this method will be implemented at the national level, resulting in faster and more citizen-friendly trials, further strengthening our fight against pandemics.

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